When a group of spins is placed in a magnetic field, each spin aligns in one of the two possible orientations. The relative numbers of spins with different alignments will be given by the Boltzmann distribution.
Definition: if a system of particles, which are able to exchange energy in collisions is in thermal equilibrium, then the relative number (population) of particles, N1 and N2, in two particular energy levels with corresponding energies, E1 and E2, is given by N1/N2 = exp [-(E1 - E2)/kT ] where k is the Boltzmann constant and T is the absolute temperature. For example, in NMR of protons at room temperature in a magnetic field of 0.25 tesla, the difference in relative numbers of spins aligned with the magnetic field and against the field is about one part in a million; the small excess of nuclei in the lower energy state is the basis of the net magnetization and the resonance phenomenon.

Bone densitometry measures the strength and density of bones. Changes in trabecular bone mineral density (BMD) is an early indicator of change in metabolic function. Bone densitometry measures the amount of calcium in regions of the bones. A bone densitometer is used to determine the risk of developing osteoporosis and can also be used to estimate a patient's risk of fracture.
Bone densitometry methods involve:
Dual energy x-rays (DEXA) or CT scans (Osteo CT or QCT) compare the numerical density of the bone (calculated from the image), with empirical data bases of bone density. DEXA is widely available and has an accuracy between those of QCT and ultrasound.

A bone marrow scintigraphy is a nuclear medical imaging procedure of the bone marrow with 99Tc-HSA-micro or nano-colloids for the early diagnostics of pathological marrow processes. The principle of this examination is the uptake of Tc-99m labeled albumin (HSA) or sulfur colloid by the reticuloendothelial cells of bone marrow. A bone marrow scan also reflects the regional blood flow. The imaging can be started 60-90 min after the injection of around 8 mCi technetium labeled colloid.

A bone scan or bone scintigraphy is used to in evaluate diseases of the skeletal system. Scintigraphic whole body bone imaging is a highly sensitive method to show changes in bone metabolism. Increased metabolic activity is seen as a hot spot.
The study requires the injection of a 99mTc-labeled radiopharmaceutical (most commonly methylene diphosphonate (MDP), hydroxymethylene diphosphonate (HMDP) or hydroxyethylene diphosphonate (HDP)). The activity administered for bone scanning is around 500 MBq (300-1100 MBq, 8-30 mCi), depending on age and weight of the patient. After 2-5 hours, the emitted gamma rays are detected by gamma cameras. The produced planar images include anterior and posterior views of the skeleton.
Multiphase bone scintigraphy is used to differentiate a bone process from tissue pathology. In some cases additional SPECT imaging is helpful to better characterize the presence, location and extent of disease.

A bone scintigraphy is a nuclear medical test used to detect increased calcium metabolisms with technetium labeled diphosphonates. Pathological bone processes present themselves primarily as activity enrichment.

See Bone Scan and Multiphase Bone Scintigraphy.